Review

pparg (Cell Signaling Technology Inc)

Cell Signaling Technology Inc is a verified supplier

Cell Signaling Technology Inc manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Cell Signaling Technology Inc pparg
Pparg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 640 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pparg+antibody/PPARgamma+Rabbit+mAb/pmc13000455-190-34-35
Average 96 stars, based on 640 article reviews

pparg - by Bioz Stars, 2026-10

96/100 stars

Images

Immunoprecipitation:

Article Title: PPARG-centric transcriptional re-wiring during differentiation of human trophoblast stem cells into extravillous trophoblasts
Article Snippet: .. For immunoprecipitation, the PPARG antibody (Cell Signaling Technology, 2443S, 1:50) was conjugated to DynabeadsTM Protein A (Thermo Fisher Scientific, 10001D) according to the manufacturer's instructions. ..

other:

Article Title: Exercise-induced adipokine Nrg4 alleviates MASLD by disrupting hepatic cGAS-STING signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Total-AKT Cell Signaling Technology Cat# 9272S; RRID: AB_329827 phospho-AKT(S473) Cell Signaling Technology Cat# 4060T; RRID: AB_2315049 phospho -TBK1 Cell Signaling Technology Cat# 5483T; RRID: AB_10693472 TBK1 Santa Cruz Biotechnology Cat# sc-52957; RRID: AB_783995 Nrg4 ABclonal Ca# 2599; RRID: AB_2764484 phospho -Erbb4 Santa Cruz Biotechnology Cat# sc-81491; RRID: AB_1125702 Erbb4 ABclonal Cat# A19047; RRID: AB_2862540 Erbb4 Santa Cruz Biotechnology Cat# sc-8050; RRID: AB_627250 STING Proteintech Cat# 19851-1-AP; RRID: AB_10665370 cGAS Proteinteh Cat# 29958-1-AP; RRID: AB_2935491 Scd1 Affinity Cat# DF13253; RRID: AB_2846272 Flag Proteintech Cat# 66008-4-Ig; RRID: AB_2918475 Pparg Cell Signaling Technology Cat# 2443; RRID: AB_823598 GAPDH Cell Signaling Technology Cat# 5174T; RRID: AB_10622025 Peroxidase affiniPure goat anti-mouse IgG secondary antibody Jackon Cat# 111-035-003; AB_10015289 Peroxidase affiniPure goat anti-rabbit IgG secondary antibody Jackon Cat# 111-035-114; AB_2307391 Bacterial and virus strains Trelief 5a Chemically Competent Cell Beijing Tsingke Biotech Cat# TSC-C01-100 Chemicals, peptides, and recombinant proteins D-glucose Sangon Biotech Cat# A610219 Human insulin Beyotime Cat# P3376-400IU Collagenase IV Sigma Cat# C5138 Oleate Sangon Biotech Cat# A502071 Palmitate Sangon Biotech Cat# A423030 Protein A/G magnetic beads Thermo Fisher Cat# 26162 Lipofectamine 6000 Beyotime Cat# C0526 IP lysis buffer Beyotime Cat# P0013 Rosiglitazone MedChemExpress Cat# HY-17386 diABZI Selleck Cat# S8796 RU.521 MedChemExpress Cat# HY-114180 Afatinib Selleck Cat# S1011 Nrg4 Protein SinoBiological Cat# 12183-HNCE Critical Commercial Assays Triglyceride Applygen Cat# E1003 Cholesterol Applygen Cat# E1005 cGAMP Cayman Cat# 501700 Genomic DNA was then extracted with a PCR purification kit Qiagen Cat# DP203 Dual Luciferase Reporter Gene Assay Kit Beyotime Cat# RG027 BeyoRTTMII First Strand cDNA Synthesis Kit Beyotime Cat# D7168M Experimental models: Cell lines HEK293T American Type Culture Collection Cat# CRL-3216 HEK293A China National Collection of Authenticated Cell Cultures Cat# SCSP-5094 3T3-L1 China National Collection of Authenticated Cell Cultures Cat# SCSP-5038 (Continued on next page) 18 Cell Reports 44, 115251, February 25, 2025

Article Title: Whey protein hydrolysates improve high-fat-diet-induced obesity by modulating the brain-peripheral axis of GLP-1 through inhibition of DPP-4 function in mice.
Article Snippet: Purpose Obesity is a growing global health concern.. Recent literature indicates a prominent role of glucagon-like peptide-1 (GLP-1) in glucose metabolism and food intake.. The synergistic action of GLP-1 in the gut and brain is responsible for its satiety-inducing effect, suggesting that upregulation of active GLP-1 levels could be an alternative strategy to combat obesity.

Article Title: PPARG directs trophoblast cell fate and establishment of the uterine-placental interface
Article Snippet: Reads from *.fastq files were mapped to the human reference genome (GRCh37) using CLC Genomics Workbench 12.0 (Qiagen).

Article Title: Exercise-induced adipokine Nrg4 alleviates MASLD by disrupting hepatic cGAS-STING signaling.
Article Snippet: Cells and tissueswere harvested, prepared andwestern blottingwas performed asdescribed previously.80,81 Lysateswere run onSDSPAGE gel (BIO-RAD) and subjected to western blotting with the primary antibodies to p-AKT(S473) (Cell Signaling Technology, Cat: 9272S, Lot:28,dilution 1:1000), tAKT (Cell Signaling Technology, Cat: 4060T, Lot:25, dilution 1:1000), p-TBK1 (Cell Signaling Technology, Cat: 5483T, Lot:28, dilution 1:1000),TBK1 (Santa Cruz Biotechnology, Cat: sc-52957, Lot:J3122, dilution 1:200), Nrg4 (ABclonal, Cat: 2599, Lot: 5500009340, dilution 1:500), p-Erbb4 (Santa Cruz Biotechnology, Cat: sc-81491, Lot:B2522, dilution 1:200), Erbb4 (ABclonal, Cat: A19047, Lot:4000002639, dilution 1:800), STING(Proteintech, Cat: 19851-1-AP, Lot:00118975, dilution 1:1000), cGAS (Proteintech, Cat: 29958-1-AP, Lot:00113331, dilution 1:800), Scd1 (Affinity, Cat:DF13253, Lot:54 3 0914, dilution 1:800), Flag (Proteintech, Cat: 66008-4-Ig, Lot:10027647, dilution 1:6000), Pparg (Cell Signaling Technology, Cat: 2443, dilution 1:1000), GAPDH (Cell Signaling Technology, Cat: D16H11, Lot: 8, dilution 1:1000), peroxidase affiniPure goat anti-mouse IgG secondary antibody (Jackon, Cat: 111-035-003, Lot: 151083, dilution 1:3000); peroxidase affiniPure goat anti-rabbit IgG secondary antibody (Jackon, Cat: 111-035-003, Lot: 153526, dilution 1:3000).

Article Title: Alistipes indistinctus-derived hippuric acid promotes intestinal urate excretion to alleviate hyperuricemia.
Article Snippet: Article Alistipes indistinctus-derived hippuric acid promotes intestinal urate excretion to alleviate hyperuricemia

Article Title: Integrative investigation on the mechanisms of modified Zuojin pill (SQQT) in ameliorating gastric metaplasia.
Article Snippet: Ethnopharmacological relevance: Zuojin pill is a well-known traditional Chinese medicine (TCM) for treating gastric disorders.. The modified Zuojin pill (SQQT) has been used in the treatment of gastric metaplasia (GM) in China for decades.. However, the mechanisms of SQQT treat GM remain unclear.

Image Search Results

Schematic of the anti-atherosclerotic mechanism of OPN-HMCN@MLT. ( A ) The study commenced with the synthesis of mesoporous carbon nanospheres (MCN) functionalized with an OPN-binding peptide and hyaluronic acid to construct the OPN-HMCN nanoplatform. The OPN-binding peptide was designed to recognize OPN enriched in the extracellular matrix and on the surface of foam cells, thereby enabling selective accumulation in OPN-rich pathological regions. Following OPN recognition, OPN-HMCN@MLT undergoes CD44-dependent endocytosis. Melatonin (MLT), a lipid autophagy–promoting agent, was subsequently encapsulated within the nanocarrier to form OPN-HMCN@MLT. Firstly, the released MLT can bind to and upregulate the expression of PPARα and PPARγ, which then promote the expression of downstream genes (ABCA1, ABCG1, ACOX-1, and CTP1A) and trigger the lipophagy. ( B ) Subsequently, its lipophagy-enhancing effects, including ABCA1/G1-mediated cholesterol efflux and CTP1A/ACOX-1-mediated mitochondrial fatty acid oxidation, were studied to confirm the reversal of foam cell formation. ( C ) These effects eventually promote foam cells to reverse into macrophages. Abbreviations: MCN, mesoporous carbon nanoparticle; OPN, osteopontin; MLT, melatonin; LDL, low-density lipoprotein; ox-LDL, oxidized low-density lipoprotein; PA, Photoacoustic.

Journal: Bioactive Materials

Article Title: A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques

doi: 10.1016/j.bioactmat.2026.02.041

Figure Lengend Snippet: Schematic of the anti-atherosclerotic mechanism of OPN-HMCN@MLT. ( A ) The study commenced with the synthesis of mesoporous carbon nanospheres (MCN) functionalized with an OPN-binding peptide and hyaluronic acid to construct the OPN-HMCN nanoplatform. The OPN-binding peptide was designed to recognize OPN enriched in the extracellular matrix and on the surface of foam cells, thereby enabling selective accumulation in OPN-rich pathological regions. Following OPN recognition, OPN-HMCN@MLT undergoes CD44-dependent endocytosis. Melatonin (MLT), a lipid autophagy–promoting agent, was subsequently encapsulated within the nanocarrier to form OPN-HMCN@MLT. Firstly, the released MLT can bind to and upregulate the expression of PPARα and PPARγ, which then promote the expression of downstream genes (ABCA1, ABCG1, ACOX-1, and CTP1A) and trigger the lipophagy. ( B ) Subsequently, its lipophagy-enhancing effects, including ABCA1/G1-mediated cholesterol efflux and CTP1A/ACOX-1-mediated mitochondrial fatty acid oxidation, were studied to confirm the reversal of foam cell formation. ( C ) These effects eventually promote foam cells to reverse into macrophages. Abbreviations: MCN, mesoporous carbon nanoparticle; OPN, osteopontin; MLT, melatonin; LDL, low-density lipoprotein; ox-LDL, oxidized low-density lipoprotein; PA, Photoacoustic.

Article Snippet: To block nonspecific binding, membranes were incubated with 5% skim milk for 1 h. Thereafter, membranes were incubated overnight at 4 °C with primary antibodies against ABCA1, ABCG1, ACOX1, CPT1A, LC3 (ab192890, 1:2000, abcam), LAMP1 (84658-5-RR, 1:8000, Proteintech), PPARα (66826-1-Ig, 1:3000, Proteintech), PPARγ (66936-1-Ig, 1:10000, Proteintech), P62 (18420-1-AP, 1:10000, Proteintech), MCAD (55210-1-AP, 1:3000, Proteintech), LCAD (17526-1-AP, 1:10000, Proteintech), tubulin (80762-1-RR, 1:10000, Proteintech), GAPDH (60004-1-Ig, 1:50000, Proteintech), and β-actin (66009-1-Ig, 1:20000, Proteintech).

Techniques: Binding Assay, Construct, Expressing

A Graphic scheme of adipogenic transdifferentiation induction protocol and microphotographs of control and transdifferentiated AsPC-1 cells at day 0, 3, 7, and 10; Rectangle dialog boxes magnify the field of view from the two groups. B Visualization of accumulated lipid droplets by oil red O staining in control and transdifferentiated AsPC-1 cells on day 10 after induction. Five random fields of view were used for quantification, values were presented as the mean ± SD ( n = 5 per group) and compared by Student’s t -test. C Relative mRNA expression levels (normalized to ACTB expression from the same individual sample) of adipocyte markers adiponectin, CEBPA, PPARG , and FABP4 in eight human pancreatic cell lines. Values were presented as the mean ± SD ( n = 3 per group) and compared by Student’s t -test. Abbreviation: SD Standard deviation.

Journal: Cell Death & Disease

Article Title: Adipogenic transdifferentiation reprograms EMT-high PDAC cells into a post-mitotic adipocyte-like state and limits metastasis

doi: 10.1038/s41419-026-08613-4

Figure Lengend Snippet: A Graphic scheme of adipogenic transdifferentiation induction protocol and microphotographs of control and transdifferentiated AsPC-1 cells at day 0, 3, 7, and 10; Rectangle dialog boxes magnify the field of view from the two groups. B Visualization of accumulated lipid droplets by oil red O staining in control and transdifferentiated AsPC-1 cells on day 10 after induction. Five random fields of view were used for quantification, values were presented as the mean ± SD ( n = 5 per group) and compared by Student’s t -test. C Relative mRNA expression levels (normalized to ACTB expression from the same individual sample) of adipocyte markers adiponectin, CEBPA, PPARG , and FABP4 in eight human pancreatic cell lines. Values were presented as the mean ± SD ( n = 3 per group) and compared by Student’s t -test. Abbreviation: SD Standard deviation.

Article Snippet: The following primary antibodies were used: polyclonal antibody against CEBPA (12968-1-AP, Proteintech, USA), polyclonal antibody against FABP4 (12801-1-AP, Proteintech), polyclonal antibody against PPARG (16643-1-AP, Proteintech), and recombinant antibody against Ki-67 (GB1514499, Servicebio, China).

Techniques: Control, Staining, Expressing, Standard Deviation

Visualization and quantification of immunostaining of CEBPA ( A ), PPARG ( B ), and FABP4 ( C ) in control and transdifferentiated AsPC-1 cells on day 10. The protein expression levels were quantified by integrated intensity and positive area of five random fields of view. Values were presented as the mean ± SD ( n = 5 per group) and compared by Student’s t -test. D The protein expression levels of CEBPA and PPARG as adipogenesis markers and GAPDH as reference marker in AsPC-1. Abbreviation: SD standard deviation.

Journal: Cell Death & Disease

Article Title: Adipogenic transdifferentiation reprograms EMT-high PDAC cells into a post-mitotic adipocyte-like state and limits metastasis

doi: 10.1038/s41419-026-08613-4

Figure Lengend Snippet: Visualization and quantification of immunostaining of CEBPA ( A ), PPARG ( B ), and FABP4 ( C ) in control and transdifferentiated AsPC-1 cells on day 10. The protein expression levels were quantified by integrated intensity and positive area of five random fields of view. Values were presented as the mean ± SD ( n = 5 per group) and compared by Student’s t -test. D The protein expression levels of CEBPA and PPARG as adipogenesis markers and GAPDH as reference marker in AsPC-1. Abbreviation: SD standard deviation.

Techniques: Immunostaining, Control, Expressing, Marker, Standard Deviation

A Schematic illustration of orthotopic PDAC models workflow. B , C Orthotopic PDAC samples collected from control and transdifferentiated groups. Tumors were outlined with yellow dashes. Tumor weight and tumor volume were presented as mean ± SD ( n = 5 per group) and compared by Student’s t -test. D Relative mRNA expression levels of adipogenesis markers (CEBPA, PPARG, FABP4, and adiponectin), EMT markers (E-cadherin and vimentin), and EMT-TFs (Snail1, Snail2, Twist1, Twist2, ZEB1, and ZEB2) in control and transdifferentiated groups. Values were presented as the mean ± SD ( n = 5 per group) and compared by Student’s t -test. E Volcano map of differentially expressed genes (DEGs) between control and transdifferentiated group ( n = 5 per group). F GO term analysis of DEGs based on gene counts showed significantly downregulated pathways in transdifferentiated group, indicating reduced angiogenesis, cellular response to cytokines, extracellular matrix organization, and cell migration. G BODIPY detection of lipid droplets in control and transdifferentiated groups from orthotopic PDAC samples. Tumors were outlined with white dashes; a representative view of fields was magnified and displayed in Fig. . Abbreviation: PDAC Pancreatic ductal adenocarcinoma, SD Standard deviation, EMT epithelial-mesenchymal transition, TF transcription factor; DEG differentially expressed genes, GO Gene ontology.

Journal: Cell Death & Disease

Article Title: Adipogenic transdifferentiation reprograms EMT-high PDAC cells into a post-mitotic adipocyte-like state and limits metastasis

doi: 10.1038/s41419-026-08613-4

Figure Lengend Snippet: A Schematic illustration of orthotopic PDAC models workflow. B , C Orthotopic PDAC samples collected from control and transdifferentiated groups. Tumors were outlined with yellow dashes. Tumor weight and tumor volume were presented as mean ± SD ( n = 5 per group) and compared by Student’s t -test. D Relative mRNA expression levels of adipogenesis markers (CEBPA, PPARG, FABP4, and adiponectin), EMT markers (E-cadherin and vimentin), and EMT-TFs (Snail1, Snail2, Twist1, Twist2, ZEB1, and ZEB2) in control and transdifferentiated groups. Values were presented as the mean ± SD ( n = 5 per group) and compared by Student’s t -test. E Volcano map of differentially expressed genes (DEGs) between control and transdifferentiated group ( n = 5 per group). F GO term analysis of DEGs based on gene counts showed significantly downregulated pathways in transdifferentiated group, indicating reduced angiogenesis, cellular response to cytokines, extracellular matrix organization, and cell migration. G BODIPY detection of lipid droplets in control and transdifferentiated groups from orthotopic PDAC samples. Tumors were outlined with white dashes; a representative view of fields was magnified and displayed in Fig. . Abbreviation: PDAC Pancreatic ductal adenocarcinoma, SD Standard deviation, EMT epithelial-mesenchymal transition, TF transcription factor; DEG differentially expressed genes, GO Gene ontology.

Techniques: Control, Expressing, Migration, Standard Deviation

Review

pparg (Cell Signaling Technology Inc)

Structured Review

Images

Related Articles

Immunoprecipitation:

other:

Similar Products

Image Search Results